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Frog Protocols
Embryology
- Animal Cap Assay
- BDA staining on section
- Dissociation and reaggregation of animal cap cells
- In Vitro Fertilization of Frog Eggs
- Hematoxylin and eosin staining
- In situ hybridization on Sections
- In Situ Hybridization to detect miRNA expression in Xenopus using LNA Probes
- Keller sandwich explant
- Natural and Artificial Fertilization of Frog Eggs
- Obtaining oocytes from frogs by surgery
- Spemann’s organizer transplantation
- Whole mount LacZ lineage tracing
- Whole mount staining with Alkaline phosphotase antibodies
- Xenopus Whole Mount Immunostaining
- Xenopus Whole Mount Immunostaining protocol 2
Molecular Biology
- Northern Blot
- RT-PCR
- Primers for RT-PCR
- Quantitative RT-PCR
- Primers for quantitative RT-PCR
- Western Blot for phosphoprotein samples
- Xenopus whole mount in situ hybridization protocol
- Xenopus laevis Morpholinos
Mouse Protocols
- AlcianBlue – Alizarin red Skeletal Staining
- Alcian Blue Cartilage Staining
- Immunohistochemistry on Mouse Sections
- In situ hybridization on mouse cryostat sections
- In situ hybridization on mouse paraffin sections
- Mallory’s tetrachrome
- Whole mount in situ hybridization on mouse embryos
Fly Protocols
- Abdominal Cuticle Preparations
- Cuticle Preparations
- Fly whole mount immunostaining
- Mounting of Adult Wings
- Wing Disc Fixation and Immunostaining
- Primers for quantitative RT-PCR
Cell Biology
- Cell Immunostainings
- Dual Luciferase Assay
- Immunostaining of dissociated Drosophila embryonic cells
- Pulse & chase labeling with 35S-methionine
- Synchronization of Cell Division
